BIOL 491 Most Recent Projects Supervised

1) PREVALENCE OF ANTIBIOTIC RESISTANCE GENES IN SEWAGE SAMPLES AT VARIOUS STAGES OF TREATMENT IN NANAIMO B.C. In this ongoing project we are examining these water samples for the presence of genes encoding for Extended Spectrum Beta lactamases (ESBLs) genes with main focus on Carbapenemases.  ESBLs confer Enterobacteria  Resistance to almost every B-lactam antibiotic available.  We are also looking of evidence of the MecA gene which gives Staphylococcus spp. resistance to methicillin which is one of the main features of MRSA. In both situations the presence of these genes in treated water might indicate that bacteria carrying these genes could been released into the environment.

Scott Britney (2017-2018)   (In progress) will continue the detection and attempt to determine the concentration of carbapenemase-producing genes blaKPC-2, blaNDM-1, and blaOXA-48 in sewage from  primary and secondary treatment the Greater Nanaimo Pollution Control Centre (co-supervised with Dr. J. Amaral)

Jeremy Kalicum (2017-2018) (In progress) will continue the detection and attempt to determine the concentration of the mecA gene in sewage from  primary and secondary treatment the Greater Nanaimo Pollution Control Centre (co-supervised with Dr. J. Amaral)

Jake Baas (2016-2017) Multiplex PCR detection of carbapenemase-producing genes blaKPC-2, blaNDM-1, and blaOXA-48 in sewage from the Greater Nanaimo Pollution Control Centre

Tomm Hailey (2016-2017) Detection of methicillin-resistant Staphylococcus spp. using multiplex PCR for genes mecA, pvl, and 16S rRNA using samples from Greater Nanaimo Pollution Control Center

Graeme Benzie (2015-2016) Detection of Carbapenemase-producing blaKPC gene in Sewage from the Greater Nanaimo Pollution Control Center (co-supervised with Dr. J. Amaral)

Dilbir Parmar (2014-2015) The Isolation of Carbapenem-Resistant Gram-negative Bacteria from Selected Water Sources in Nanaimo, B.C., Canada (co-supervised with Dr. J. Amaral)

2) ISOLATION OF ANTIOBIOTIC RESISTANT AND “ANTIBACTERIAL PRODUCT” RESISTANT STRAINS OF STAPHYLOCOCCUS spp FROM COMMUNITIES IN VANCOUVER ISLAND We had been collecting Staphylococcus  isolates since 2003  by swabbing public places (phones, ATM machines, grocery carts, door handles etc.), homeless shelters, needle exchange clinics, and many other locations in Nanaimo, Victoria, and Campbell River.  We had characterize the isolates and tested them in the lab for antibiotic resistance and resistance to antimicrobial products. At this point we have a collection of about 100 isolates which had been characterize for the antibiotic resistace profile and their sensitivity to selected antimicrobial agents.  Several students had been contributing to this project since the beginning:

Tristan Douglas (2014-2015) Frequency of Methicillin-Resistant Staphylococcus spp. Colonization among Nursing Students at Vancouver Island University, Nanaimo, British Columbia, Canada

Cole Van Pystra (2013-2014) ” Prevalence of methicillin-resistance and Panton-Valentine Leukocidin genes in Staphylococcus spp. isolates from anterior nares of students from Vancouver Island University, Nanaimo, British Columbia”

Colten Linterman (2012-1013) ” Prevalence of Panton-Valentine Leukocidin Genes in Methicillin-Resistant Staphylococcus aureus and Methicillin-Resistant Coagulase-Negative Staphylococci Isolates from Central and South Vancouver Island, British Columbia”

 

OTHER BIOL 491 RESEARCH PROJECTS THAT I HAD RECENTLY SUPERVISED: 

Shelby Reimer (2016-2017) Inhibition of Bacterial Quorum Sensing by Ibuprofen in Chromobacterium violaceum CV026

Amanda Bibby. (2013-2014) Detection of Anammox Bacteria in Recirculating Aquaculture Systems using Nested PCR (co-supervised with Dr. J. Amaral)

Jenna Langill. (2013-2014)  The Effect of D-Amino Acids on Biofilms of Streptococcus mutans and Streptococcus salivarius (co-supervised with Dr. J. Amaral)

 

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